progress
specimens barcoded:  19243
 
species barcoded:  2779
 
unnamed barcode  
clusters found: 
858
 
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Lab Procedures
Lab protocols
    Once Matrix boxes and data arrive at the CCDB, specimen information is transferred onto BOLD. Specimens are sub-sampled, i.e. tissue (in most cases, one leg) is removed from the specimen and placed into a well in a lysis plate. The remainder of the specimen is stored in the original tube and deposited at the CCDB or sent back to the collaborator after being photographed. Tissue samples subsequently go through a high-throughput lab procedure that includes DNA extraction, PCR, cycle sequencing, and sequencing. The project manager oversees the whole procedure. Depending on the success rate of a particular plate of samples, positive hit picking of PCR products or negative hit picking of failures can be done on automatic robots. The turn-around time for the lab work is normally 1-2 weeks. The CCDB has the capacity to process 500,000 specimens each year.

Data sharing and analyses via BOLD
    All collaborators will be registered as project users on BOLD, providing them with direct access to the management console for their project, making it possible to monitor progress, and to run analysis using tools provided in BOLD before the results are available publicly. Additionally, Trichoptera systematists can edit specimen information, e.g., updating taxonomy directly on the webpage. BOLD serves not only as a data depository, but also a communication platform between barcoders and collaborators. For example, the taxonomic browser that is integrated in BOLD will show what species have been barcoded and what species are needed.

    Trichoptera barcodes will eventually be published and become publicly accessible. At the meantime, barcodes will be submitted to GenBank and cross-linked on both websites.

Post-sequencing
    Collecting barcodes for trichopterans is not the only goal of the barcoding initiative. Almost certainly, DNA barcodes will indicate some very interesting questions that might have been overlooked by conventional approaches. For instance, cryptic species that share nearly identical morphology can be readily separated into distinct groups on the DNA tree. Other "species" may need to be synonymized. As a general rule, all cases of conflict between the DNA tree and morphological assignment deserve additional investigation. DNA barcoders will continue working with trichopterologists, ecologists, and many other researchers after DNA sequences are generated.